Corning® Epidermal Growth Factor (EGF), mouse natural (culture grade),100µg, is a low-molecular-weight mitogenic protein that stimulates proliferation of a wide variety of cell types in vitro. 1/pack. These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.
Formulation
Lyophilized from ammonium acetate, carrier-free; reconstitute in dH2O
Preparation and Storage
Stable for at least three months at 2° to 8°C (lyophilized) or = two months at -20°C (solubilized). Do not store in frost-free freezer. Avoid multiple freeze/thaws.
Quality
- Purity: >95% by SDS-PAGE and >80% by pH 9.9 PAGE
- Tested for ability to stimulate proliferation of human foreskin fibroblasts in serum-containing medium
- Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
Source
- Male mouse submaxillary glands
- Molecular Weight
- 6.1 kD
Tip
Recommended concentration: 5 to 100 ng/ mL medium depending on cell type
Storage
Shelf life: >= 3 months from date of shipment at 2-8°C (lyophilized) or >= two months at -20°C (solubilized)
