Corning® Epidermal Growth Factor (EGF), mouse natural (receptor grade), 100µg, is a low-molecular-weight mitogenic protein that stimulates proliferation of a wide variety of cell types in vitro. 1/pack These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.
Formulation
Lyophilized from ammonium acetate, carrier-free; reconstitute in dH2O
Preparation and Storage
Stable for at least three months at 2° to 8°C (lyophilized) or = one month solubilized at -20°C . Do not store in frost-free freezer. Avoid multiple freeze/thaws.
Quality
- Purity: >98% by SDS-PAGE and pH 9.9 PAGE
- Tested for ability to stimulate proliferation of human foreskin fibroblasts in serum-containing medium
- Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, and mycoplasma
Source
Male mouse submaxillary glands
Molecular Weight
6.1 kD
Purification
Purified by BioGel® P10/DEAE chromatography
Tip
Recommended concentration: Approximately 5 ng/ mL medium depending on cell type
Storage
Shelf life: >= 3 months from date of shipment at 2-8°C (lyophilized) or >= one month at -20°C (solubilized)
