Corning® 500 mL CMRL 1066

without L-glutamine A less complex and extensively modified version of Medium 199, CMRL-1066 was designed initially for use with L-strain cells in unsupplemented culture. It has been used to maintain monolayer growth of permanent cell lines for years without protein supplementation. Although developed for use in serum-free cell culture, CMRL-1066 can be supplemented with serum and used to support the growth of many types of cells.