Corning® 500 mL Graces Insect Basal Medium (Vaughn Mod.) [+] L-glutamine, [-] in

with L-glutamine, without insect hemolymph The insect cell culture medium was formulated to closely resemble insect hemolymph, a compound crucial to the function of the insect circulatory system.  The basal medium, when properly supplemented, has been used to culture cells derived from a variety of insects including several species of lepidopterans as well as some dipterans.  The medium is primarily used as a basal medium for the growth and maintenance of cell lines derived from lepidopterans.